Uncovering RNA-Protein Interactions with RNA Antisense Purification and Mass Spectrometry (RAP-MS)
What is RAP-MS? RNA molecules play a vital role in cellular functions, ranging from gene expression regulation to protein synthesis. Understanding the interactions between RNA and proteins is crucial to comprehend the intricate mechanisms of cellular biology. However, identifying RNA-protein interactions can be challenging, especially for long noncoding RNAs (lncRNAs) that do not encode proteins. RNA antisense…
dorawestChIRP and ChIRP-Seq Techniques in Studying Gene Expression Regulation
The intricate interplay between DNA and histone proteins results in the formation of chromatin, which performs a pivotal function in governing the expression of genes. The dynamic process of chromatin remodeling and modifications plays a decisive role in regulating the accessibility of DNA to the transcriptional machinery, thereby leaving an indelible imprint on gene expression….