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dorawest12

Proximity Labeling Techniques

Proximity Labeling Techniques

Proximity labeling works by fusing the decoy protein to be studied with an enzyme that nonspecifically labels the surrounding protein. The tagged proteins are called “prey” proteins. There is a rapid development of protein labeling techniques based on proximity-tagging enzymes. Biotin-dependent biotin identification (BioID) of biotin ligase mutants relying on biotin ligase (BirA), which can label lysine…

RNA-Protein Interactions with ChIRP-MS

RNA-Protein Interactions with ChIRP-MS

Explore the diverse applications of Chromatin Isolation by RNA Purification and Mass Spectrometry (ChIRP-MS) in understa